Extraction process of pancrelipase and evaluation thereof
AssigneeKashiv Biosciences, LLC
InventorsRaja Reddy Kuppili, Chintan Mineshkumar Vaywala, Parva Yogeshchandra Purohit, Mukesh Mahajan, Sanjaykumar Vanrajbhat Talpara
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Abstract
The invention provided herein a pharmaceutical process used for an extraction of proteins from pancreatic sample and estimation of the extracted proteins. Moreover, the invention provides a use of suitable is selected from citrate-phosphate buffer and bicarbonate buffer capable to extract proteins from pancreatic sample. The invention further provides an analytical method to perform estimation of extracted proteins. This process provides an improved extraction method to quantify protein present in the pancreatic sample.
Claims20
A process for an extraction of a pancreatic protein from a pancreatic protein sample, the process comprising:
- a. treating the pancreatic protein sample with a buffer;
- b. dissolving the pancreatic protein sample in the buffer;
- c. collecting the extracted pancreatic protein,
- wherein the suitable buffer is selected from citrate-phosphate buffer and bicarbonate buffer;
- wherein the pancreatic protein sample comprises a pharmaceutical composition comprising:
- one or more enzymes selected from lipase, protease, and amylase;
- and
- one or more enzymes selected from Triacylglycerol lipase, Co-lipase, CEL lipase, Phospholipase A2, Trypsin, Chymotrypsin, Elastase, Carboxypeptidase A1, Carboxypeptidase B, Kallikrien glandular, and Alpha amylase.
Dependencies are inferred from the claim text.