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US 12,710,423 B2Granted
African swine fever DIVA immunoassay
AssigneeIntervet Inc.
InventorsErwin Van Den Born, Urs Peter Bruderer
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Abstract
The invention relates to a diagnostic use of an African Swine Fever Vims CD2v protein, a method, a device, and a kit for the detection of the presence of ASFV antibodies in a test sample, in particular the use thereof in a DIVA immunoassay.
Claims14
A method for distinguishing if a swine is infected with a wild-type ASFV or vaccinated with an ASFV live attenuated virus CD2v-marker vaccine (LAV CD2v-marker vaccine), the method comprising the steps of:
- adding a test sample obtained from the swine with an antigen, wherein the antigen is an isolated antigenic fragment of a ASFV CD2v protein and wherein the antigenic fragment is a polypeptide comprising an amino acid sequence with at least 95% amino acid sequence identity to SEQ ID NO:
- 25,
- incubating the sample with the antigen for a sufficient amount of time to allow the formation of an antibody-antigen complex, comprising an antibody from the test sample and the antigen,
- detecting the presence or absence of the antibody-antigen complex, and
- wherein the presence of the antibody-antigen complex identifies the swine from which the test sample is obtained as being infected with a wild-type ASFV, and the absence of an antibody-antigen complex identified the swine as vaccinated with a LAV CD2v-marker vaccine.
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Dependencies are inferred from the claim text.