Extracellular vesicle characterization systems
AssigneeMursla Limited
InventorsPierre Arsene, Tomas Miguel De Freitas Dias
Life of this patent
Estimating the term…
Abstract
A method of determining a number of epitopes on an exosome using a combination of optical and electrical interrogation techniques. Implementations of the method quantify the number of epitopes on a target exosome, e.g. a tumour-derived exosome.
Claims34
A method of detecting binding sites on an extracellular vesicle, comprising:
- obtaining a liquid sample containing extracellular vesicles;
- attaching reporters to binding sites on the extracellular vesicles;
- and
- optically interrogating the reporters to characterize a number of bindings of the reporters to the extracellular vesicles;
- and
- wherein attaching reporters to binding sites on the extracellular vesicles comprises:
- providing a detection system comprising a binding site recognition element linked to biotin and a biotin-binding protein attached to the reporter;
- and
- allowing the liquid sample containing the extracellular vesicles to interact with the detection system.
A method of determining a number of binding sites on an extracellular vesicle, comprising:
- obtaining a liquid sample containing extracellular vesicles;
- attaching electrically conducting nanoparticles to the extracellular vesicles;
- attaching reporters to binding sites on the extracellular vesicles;
- interrogating the reporters to determine a total number of bindings of the reporters to the extracellular vesicles;
- determining a number of extracellular vesicles in the liquid sample by sensing an electrical response of the liquid sample using a pair of electrodes separated by less than an average maximum dimension of the electrically conducting nanoparticles;
- and
- combining the determined total number of bindings and the determined number of extracellular vesicles in the liquid sample to determine a number of binding sites per extracellular vesicle.
A system for detecting binding sites on an extracellular vesicle, wherein the system is configured to:
- accept a liquid sample containing extracellular vesicles;
- attach reporters to binding sites on the extracellular vesicles;
- and
- optically interrogate the reporters to characterize a number of bindings of the reporters to the extracellular vesicles;
- and
- wherein attaching reporters to binding sites on the extracellular vesicles comprises:
- providing a detection system comprising a binding site recognition element linked to biotin and a biotin-binding protein attached to the reporter;
- and
- allowing the liquid sample containing the extracellular vesicles to interact with the detection system.
A system for determining a number of binding sites on an extracellular vesicle, wherein the system is configured to:
- accept a liquid sample containing extracellular vesicles;
- attach electrically conducting nanoparticles to the extracellular vesicles;
- attach reporters to binding sites on the extracellular vesicles;
- interrogate the reporters to determine a total number of bindings of the reporters to the extracellular vesicles;
- determine a number of extracellular vesicles in the liquid sample by sensing an electrical response of the liquid sample using a pair of electrodes separated by less than an average maximum dimension of the electrically conducting nanoparticles;
- and
- combine the determined total number of bindings and the determined number of extracellular vesicles in the liquid sample to determine number of binding sites per extracellular vesicle.
Dependencies are inferred from the claim text.