US 12,710,405 B1 · Kit for detecting drug in sample and detection method thereof

Abstract

The present application provides a kit for therapeutic drug monitoring, and a method and system thereof. A magnetic bead extraction method combined with LC-MS/MS detection is applied to drug concentration monitoring in human blood. A mixed magnetic bead solution containing a phospholipid depletion magnetic bead and a to-be-detected drug-adsorption magnetic bead is used for adsorption and extraction pretreatment of a drug to be detected in a sample. In conjunction with automated treatment equipment, the pretreatment efficiency is high such that the content of phospholipids in a supernatant is low, thereby greatly reducing the matrix effect and the chromatographic column flushing pressure. The method for therapeutic drug monitoring provided by the present application uses a unified pretreatment flow to achieve simultaneous extraction of multiple drugs. Moreover, by means of an automated magnetic bead extraction method, the steps for sample pretreatment can be completed rapidly, and the results for therapeutic drug monitoring can be obtained rapidly, such that the overall detection time is shortened, thereby providing a reliable laboratory examination basis for achieving individualized therapy.

Claims4

Dependent claims

A method for drug detection, comprising the following steps:

making a mixed magnetic bead solution in contact with a sample, wherein the mixed magnetic bead solution comprises a magnetic bead for removing phospholipids in the sample and a magnetic bead for adsorbing a drug to be detected in the sample, such that the phospholipids and the drug to be detected in the sample are simultaneously adsorbed;
and
eluting, with an eluent, the drug to be detected from the magnetic bead for adsorbing the drug to be detected in the sample, and analyzing a content of the drug to be detected by liquid chromatography-tandem mass spectrometry,
wherein the drug to be detected comprises any one or more of a sedative-hypnotic drug, an antidepressant drug, an antipsychotic drug, an antiepileptic drug, an antibiotic drug, an antineoplastic drug, a cardiovascular drug, and a toxicology screening drug;
wherein the magnetic bead for removing the phospholipids in the sample comprises a ZrO2 silica magnetic bead, and the magnetic bead for adsorbing the drug to be detected in the sample comprises an HLB magnetic extraction bead;
and
wherein the mixed magnetic bead solution comprises an acid, and the acid comprises any one or more of formic acid, acetic acid, and citric acid.

4 more elements

Dependencies are inferred from the claim text.

Kit for detecting drug in sample and detection method thereof

Life of this patent

Kit for detecting drug in sample and detection method thereof

Life of this patent

Abstract

Claims4