Optical flow cytometer for fluorescence and scattering measurements by segmentation of beam emitted by a single non-coherent light source

An optical flow cytometer for fluorescence measurement and scattering measurement comprising:

• a non-coherent light source intended to generate an illumination beam;
• and
• a beam truncation device comprising at least:
• a first passage to let pass a first part of the illumination beam with a first divergence, the first part being dedicated to scattering measurements;
• a second passage to let pass a second part of the illumination beam with a second divergence, the second part being dedicated to fluorescence measurements;
• the second divergence being greater than the first divergence;
• the first and second passages being separated by an area of the beam truncation device that stops the illumination beam;
• at least a focalization lens to focus said first part and said second part of the illumination beam on a flowcell comprising an optical interrogation zone;
• the flowcell intended to contain particles flowing through the optical interrogation zone;
• the beam truncation device and the at least a focalization lens are optically positioned between the light source and the flowcell;
• a scattering detector for receiving light that is scattered from the first part of the illumination beam as a particle crosses the optical interrogation zone;
• and
• a fluorescent detector for receiving fluorescent light emitted by the particle crossing the optical interrogation zone.