Methods and systems of determining a viscosity of liquid samples

A method for determining a viscosity of a sample of a protein, the method comprising:

• positioning the sample of the protein in a first location of an acoustic liquid handler;
• applying, using the acoustic liquid handler, one or more first acoustic signals until a specified amount of the sample has been transferred from the first location to a second location of the acoustic liquid handler;
• and
• determining the viscosity of the sample based on a number of the one or more first acoustic signals required to transfer the specified amount of the sample from the first location to the second location,
• wherein the positioning includes positioning the sample of the protein in a first well of the first location of the acoustic liquid handler, and wherein the applying includes applying the one or more acoustic signals until the specified amount of the sample has been transferred from the first well of the first location to a second well of the second location of the acoustic liquid handler.

A method for determining a viscosity of a sample of a protein, the method comprising:

• positioning the sample of the protein in a first location of an acoustic liquid handler;
• applying, using the acoustic liquid handler, one or more first acoustic signals until a specified amount of the sample has been transferred from the first location to a second location of the acoustic liquid handler;
• and
• determining the viscosity of the sample based on a number of the one or more first acoustic signals required to transfer the specified amount of the sample from the first location to the second location,
• wherein determining the viscosity of the sample further comprises determining the viscosity of the sample based on a set of parameters of the first acoustic signals, wherein the set of parameters includes at least two of a frequency, a power, an amplitude, a wavelength, a bandwidth, and a period, and wherein the one or more first acoustic signals each have a frequency in the range of 1 mHz to 5 mHz.

A method for determining a viscosity of a sample of a protein, the method comprising:

• positioning the sample of the protein in a first location of an acoustic liquid handler;
• applying, using the acoustic liquid handler, one or more first acoustic signals until a specified amount of the sample has been transferred from the first location to a second location of the acoustic liquid handler;
• determining the viscosity of the sample based on a number of the one or more first acoustic signals required to transfer the specified amount of the sample from the first location to the second location;
• and
• generating mass spectrometric data on the sample, wherein the one or more first acoustic signals transfer the sample or a portion of the sample thereof to a mass spectrometer.

A method for determining a viscosity of a sample of a protein, the method comprising:

• positioning the sample of the protein in a first location of an acoustic liquid handler;
• applying, using the acoustic liquid handler, one or more first acoustic signals until a specified amount of the sample has been transferred from the first location to a second location of the acoustic liquid handler;
• determining the viscosity of the sample based on a number of the one or more first acoustic signals required to transfer the specified amount of the sample from the first location to the second location;
• and
• determining whether the specified amount of the sample has been transferred,
• wherein determining whether the specified amount of the sample has been transferred comprises:
• applying one or more second acoustic signals to a first portion of the sample not transferred from the first location;
• determining an amount of the first portion of the sample not transferred based on the application of the one or more second acoustic signals;
• determining an amount of a second portion of the sample transferred based on the determining of the amount of the first portion of the sample not transferred;
• and
• comparing the amount of the second portion of the sample transferred and/or the first portion of the sample not transferred to the specified amount of the sample to be transferred.