Puccinia resistance gene
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsEvans Lagudah, Jianping Zhang, Peng Zhang
Life of this patent
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Abstract
The present invention relates to a plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to at least one strain of Puccinia graminis.
Claims19
A genetically modified plant comprising a transgene encoding a polypeptide which confers resistance to at least one strain of Puccinia graminis, wherein the polypeptide comprises amino acids having a sequence as provided in SEQ ID NO:1, or an amino acid sequence which is at least 95% identical to SEQ ID NO:1.
A process for identifying a polynucleotide encoding a polypeptide which confers resistance to at least one strain of Puccinia graminis comprising:
- i) obtaining a polynucleotide operably linked to a promoter, the polynucleotide encoding a polypeptide comprising amino acids having a sequence as provided in SEQ ID NO:1, or an amino acid sequence which is at least 9% identical to SEQ ID NO:1,
- ii) introducing the polynucleotide into a plant by transgenesis,
- iii) determining whether the level of resistance to Puccinia graminis is modified relative to an isogenic plant lacking the polynucleotide, and
- iv) optionally, selecting a polynucleotide which when expressed confers resistance to Puccinia graminis.
A method for identifying a plant comprising a polynucleotide encoding a polypeptide which confers resistance to at least one strain of Puccinia graminis, the method comprising the steps of
- i) obtaining a nucleic acid sample from a plant, and
- ii) screening the sample for the presence or absence of the polynucleotide encoding a polypeptide which confers resistance to at least one strain of Puccinia graminis, wherein the polypeptide comprises amino acids having a sequence as provided in SEQ ID NO:1, or an amino acid sequence which is at least 95% identical to SEQ ID NO:1, using a method selected from nucleic acid amplification, nucleic acid sequencing, nucleic acid hybridization with suitably labelled probes, single-strand conformational analysis (SSCA), denaturing gradient gel electrophoresis (DGGE), heteroduplex analysis (HET), chemical cleavage analysis (CCM), catalytic nucleic acid cleavage or a combination thereof.
Flour or wholemeal comprising a transgene encoding polypeptide which confers resistance to at least one strain of Puccinia graminis, wherein the polypeptide comprises amino acids having a sequence as provided in SEQ ID NO:1, or an amino acid sequence which is at least 95% identical to SEQ ID NO:1.
Dependencies are inferred from the claim text.