Rapid design, build, test, and learn technologies for identifying and using non-viral carriers

A composition comprising:

• a non-viral delivery vehicle comprising one or more nanoparticle forming polymers, and
• a nucleic acid construct, comprising:
• two primer binding segments;
• and
• one or more unique polynucleotide barcodes between the two primer binding segments,
• wherein the nucleic acid construct is:
• electrostatically associated with the nanoparticle forming polymers;
• bonded to the nanoparticle forming polymers via complexation of biotin and a biotin binding molecule;
• or
• covalently bonded to the nanoparticle forming polymers,
• wherein the one or more nanoparticle forming polymers are RAFT block copolymers comprising:
• a. a first terminus comprising a first capping unit derived from a first chain transfer agent in a RAFT copolymerization process;
• b. a first block prepared from one or more monomer units covalently attached to the first reactive functional unit, and having a molecular weight (Mn) in the range of about 1 kDa to about 200 kDa and a degree of polymerization in the range of about 10 to about 2500;
• c. optionally a second block prepared from one or more monomer units covalently attached to the first block, and having a molecular weight (Mn) in the range of about 1 kDa to about 200 kDa and a degree of polymerization in the range of about 20 to about 2000;
• and
• d. a second terminus comprising a second capping unit derived from a first or a second chain transfer agent.