Adapting control of a cell culture in a production scale vessel with regard to a starting medium

A system for adapting control of a cell culture in a production-scale vessel with regard to a starting medium, the system comprising:

• a database storing production-scale process trajectories, each derived from a successfully controlled cell culture;
• a first process-control device for controlling the cell culture in the production-scale vessel, the first process-control device comprising:
• the production-scale vessel, configured to receive first media from a media lot;
• a first controller operable to receive output from a seed train started using the first media in order to achieve inoculation of the production-scale vessel;
• a second process-control device, the second process-control device comprising:
• a plurality of micro-scale vessels, each of the micro-scale vessels being configured to contain a representative portion of the media lot, wherein the production-scale is greater than the micro-scale;
• a robot capable of addressing each of the vessels, dispensing fluid to each of the vessels, and extracting samples of fluid from each of the vessels;
• and
• a second controller comprising a processor and a non-transitory data storage device storing instructions that, when executed by the processor, cause the second controller to:
• distribute second media sampled from the media lot to the micro-scale vessels;
• receive cells from the seed train for the micro-scale vessels to start cell cultures in each of the micro-scale vessels;
• control and monitor, at least partly in parallel, the cell cultures in each of the micro-scale vessels;
• determine, during the cell culture run or at the end of the cell culture run for the micro-scale vessels, process trajectories from each one of the micro-scale vessels;
• determine a statistically representative trajectory from the process trajectories of the micro-scale vessels, wherein the statistically representative trajectory represents an impact of the second media on the cell cultures;
• determine whether the first media is suitable for controlling the cell culture in the production-scale vessel based on the impact of the second media on the cell cultures;
• and
• after determining that the first media is suitable:
• compare the statistically representative trajectory to the stored production-scale process trajectories;
• and
• control the cell culture of the production-scale vessel, using the first media, based on the comparison,
• wherein the comparing comprises calculating a similarity of the trajectories, wherein the similarity of the trajectories is calculated according to a multivariate distance measure.