Polymeric surface having reduced biomolecule adhesion to thermoplastic articles of such substrate

A method for providing a non-specific biomolecule adsorption and/or non-specific biomolecule binding resistant coated surface, the method comprising the steps:

• (a) providing a substrate with a prepared surface;
• (b) reacting the prepared surface with at least one zwitterionic copolymer, thus forming a non-specific biomolecule adsorption and/or biomolecule binding resistant coated surface;
• wherein reacting the prepared surface with at least one zwitterionic copolymer comprises:
• reacting the prepared surface with a vapor comprising at least one silyl amine coupling agent to provide an amine modified surface;
• contacting the amine modified surface with a copolymer solution comprising a solvent, wherein the copolymer comprises at least one copolymerizable zwitterionic monomer and at least one copolymerizable epoxy monomer, optionally in the presence of a polymerization initiator;
• and
• wherein the at least one copolymerizable epoxy monomer is selected from the group consisting of (E)-(oxiran-2-yl)methyl but-2-enoate, (3,3-dimethyloxiran-2-yl)methyl methacrylate, (E)-(oxiran-2-yl)methyl cinnamate, (oxiran-2-yl)methyl 2-methylenebutanoate, 1-(oxiran-2-yl) propyl acrylate, 1-(oxiran-2-yl)ethyl methacrylate, (oxiran-2-yl)methyl 3-methyl-2-methylenebutanoate, (oxiran-2-yl)methyl 2-methylenepentanoate, (3-methyloxiran-2-yl)methyl acrylate, 2-(oxiran-2-yl) propan-2-yl acrylate, (oxiran-2-yl)methyl 2-methylenehexanoate, (3-methyloxiran-2-yl)methyl methacrylate, (3,3-dimethyloxiran-2-yl)methyl acrylate, and combinations thereof.