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US 12,709,645 B2Granted
Targeting gene amplification in cancer using triplex formation as a therapeutic strategy
AssigneeYale University
InventorFaye A. Rogers
Life of this patent
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Abstract
Disclosed herein are methods and agents for the treatment of cancer using p53-independent apoptosis to reduce the number of p53-depleted or p53-mutated cancer cells that have amplified HER2 gene. Also disclosed herein are methods and agents for the treatment of HER2-positive cancer in individuals with Li-Fraumeni Syndrome.
Claims9
A method of inducing DNA damage in cancer cells, the method comprising contacting the cancer cells with a first triplex forming oligonucleotide (TFO) and a second TFO, wherein:
- (a) the cancer cells comprise an amplified HER2 gene that comprises a first polypurine target site and a second polypurine target site that is different from the first polypurine target site,
- (b) the first TFO is complementary to and hybridizes to the first polypurine target site in the amplified HER2 gene, and the second TFO is complementary to and hybridizes to the second polypurine target site in the amplified HER2 gene, and
- (c) hybridization of the first TFO to the first polypurine target site and the second TFO to the second polypurine target site in the amplified HER2 gene induces DNA damage in the cancer cells.
Dependencies are inferred from the claim text.