Flow control mechanism for high-throughput oligonucleotide synthesis

An apparatus comprising:

• a pressurized chamber;
• and
• an equilibrated oligonucleotide synthesis (EOS) system inside the pressurized chamber, the EOS system comprising:
• four or more synthesis vessels, each of the four or more synthesis vessels having a waste emitting end;
• a synthesis plate holding the four or more synthesis vessels, the synthesis plate having a bottom surface with grooves;
• a drain block located below and operably coupled to the synthesis plate;
• a sealing device disposed between and in contact with the synthesis plate and the drain block, the sealing device having holes to allow flow of material from the four or more synthesis vessels to the drain block;
• one or more waste collection reservoirs located inside of the drain block;
• one or more equilibration holes, each of the one or more equilibration holes penetrating an exterior of the drain block and one of the one or more waste collection reservoirs, the one or more equilibration holes located above the waste emitting end of each of the four or more synthesis vessels;
• and
• one or more waste tubes each of the one or more waste tubes operably connected to the one or more waste collection reservoirs.

An apparatus comprising:

• a pressurized chamber;
• and
• an equilibrated oligonucleotide synthesis (EOS) system inside the pressurized chamber, the EOS system comprising:
• a plurality of synthesis vessels, each of the plurality of synthesis vessels having a waste emitting end;
• one or more sealing devices, each of the one or more sealing devices comprising one or more sealing blocks, each of the one or more sealing blocks holding one or more synthesis vessels of the plurality of synthesis vessels and each of the one or more sealing blocks having at least one equilibration hole, the at least one equilibration hole located above the waste emitting end of the one or more synthesis vessels;
• a drain block located below and operably coupled to the one or more sealing blocks, the drain block comprising a plurality of machined plates, each machined plate of the plurality of machined plates comprising a set of grooves;
• and
• one or more waste tubes each of the one or more waste tubes operably connected to at least one of the plurality of machined plates.