Selective modulation of intracellular effects of cells using pulsed electric fields

A non-thermal ablation system comprising:

• at least one electrode;
• a voltage pulse generator operatively coupled to the at least one electrode and configured to apply a plurality of electrical pulses to a target region with a delay between successive pulses, wherein at least one pulse parameter relative to the plurality of pulses is optimized to produce a first treatment zone and a second treatment zone;
• wherein only selected cells are affected by the plurality of electrical pulses in the second treatment zone;
• and
• wherein the voltage pulse generator is configured to apply the plurality of electrical pulses in a manner such that non-thermal ablation occurs in the first treatment zone.

A non-thermal ablation system comprising:

• two or more electrodes;
• a voltage pulse generator operatively coupled to the two or more electrodes and configured to apply a plurality of electrical pulses to a target region with a delay between successive pulses, wherein the plurality of pulses is configured to produce a first treatment zone and a second treatment zone;
• wherein only selected cells are affected by the plurality of electrical pulses in the second treatment zone;
• and
• wherein the voltage pulse generator is configured to apply the plurality of electrical pulses in a manner such that non-thermal ablation occurs in the first treatment zone.