Birth tissue compositions and methods of preparation

A method of treating a wound, the method comprising the step of:

• introducing a birth tissue composition on or around the wound,
• wherein the birth tissue composition comprises a dehydrated, decellularized porcine placental membrane that is prepared by a method comprising:
• (i) a bioburden reduction step comprising contacting unprocessed porcine placental membrane with a 3M sodium chloride solution at about 20 mL per gram of porcine placental membrane for about 30 minutes to about 2 hours to reduce microbial load and form a bioburden reduced porcine placental membrane;
• (ii) decanting the sodium chloride solution;
• (iii) rinsing the sodium chloride solution from the bioburden reduced porcine placental membrane with water;
• (iv) a detergent rinse step comprising contacting the bioburden reduced porcine placental membrane after step (iii) with a detergent solution comprising at least one anionic detergent and at least one protease enzyme, wherein the anionic detergent is present at a concentration of about 0.25% to about 3% w/v, wherein the contacting occurs for about one hour to about three hours, for removal of cellular debris while preserving extracellular matrix integrity;
• and
• (v) decanting the detergent solution to form a detergent rinsed porcine placental membrane;
• (vi) rinsing the detergent solution from the detergent rinsed porcine placental membrane with water;
• (vii) a viral inactivation step comprising immersing the rinsed porcine placental membrane after step (vi) in a sodium hydroxide solution at a concentration of about 0.25M for about 15 minutes to about 45 minutes to form a viral inactivated porcine placental membrane;
• (viii) rinsing the sodium hydroxide solution from the viral inactivated porcine placental membrane with water;
• (ix) introducing from about 5 mL to about 50 mL of buffer solution per gram of porcine placental membrane to the rinsed porcine placental membrane after step (viii);
• (x) decanting the buffer solution;
• (xi) measuring the pH of the porcine placental membrane after step (x);
• (xii) repeating steps (ix)-(xi) until the pH of the porcine placental membrane is between about 6.8 and about 7.2;
• (xiii) rinsing the buffer solution from the porcine placental membrane after step (xii) with water;
• and
• (xiv) dehydrating the porcine placental membrane after (xiii) to form the dehydrated, decellularized porcine placental membrane,
• wherein the dehydrated, decellularized porcine placental membrane exhibits a final pH of between about 6.8 and about 7.2, and
• wherein the dehydrated, decellularized porcine placental membrane retains collagen I, collagen IV, elastin, laminin, fibronectin, and hyaluronic acid.