Delivery of nucleic acids, proteins and small molecules in vitreous vesicular bodies

A composition comprising:

• one or more isolated, aqueous humor and/or vitreous humor extracellular vesicle bodies, wherein said extracellular vesicle bodies are modified in vitro to contain one or more exogenous agents, wherein endogenous contents of said extracellular vesicle bodies are removed, wherein said extracellular vesicle bodies are from 100 to 6,000 nanometers in diameter, wherein the composition is at least 75% free of cell or cellular debris, and wherein said extracellular vesicle bodies are modified to display a eukaryotic cell-specific targeting molecule on the vesicle surface so that the composition mediates uptake of the one or more exogenous agents by the eukaryotic cells, wherein the eukaryotic cells are selected from the group consisting of human skin cells, human retinal cells, human ciliary cells, and human corneal cells.