Digital health system for the continuous quantification of physiological biomarkers, biological regulators, and analytes in real-time using optical quantification

A device, comprising:

• a casing comprising an entry port;
• a biological-assay cartridge comprising a housing:
• the entry port being adapted to receive the biological-assay cartridge;
• the biological-assay cartridge being configured to receive a biological sample comprising analytes wherein the biological-assay cartridge comprises a sampling port for obtaining the biological sample, the sampling port being located outside the housing, a plunger shaft for creating a vacuum to obtain the biological sample through the sampling port, a vacuum port configured to cause the flow of the biological sample into a channel within the cartridge, and a flowrate check valve configured to control the flowrate of the biological sample into the channel;
• contact sensors formed on an outer portion of the casing;
• and
• a biological-assay reader placed inside the casing configured to read the biological sample, wherein the biological-assay reader comprises:
• at least one light source for directing light onto the biological sample;
• and
• at least one light detector for identifying a concentration of an analyte in the biological sample by analyzing light emitted from the biological sample in response to the light directed from the source.