Back to results
US 12,708,299 B2Granted
Direct electron transfer glutamate biosensor using platinum nanoparticle and carbon nanotubes
AssigneePurdue Research Foundation
InventorsHyowon Lee, Tran N.H. Nguyen
Life of this patent
Estimating the term…
Abstract
A direct electron transfer amperometric biosensor fabricated using direct write printing technology for in vivo electrochemical monitoring, such as monitoring of neurotransmitters and other biomarkers, e.g., in traumatic spinal cord injury. The biosensor is fabricated by immobilizing glutamate oxidase on nanocomposite electrodes made of platinum nanoparticles, multiwall carbon nanotubes and a conductive polymer on a flexible substrate.
Claims20
An implantable biosensor comprising a nanocomposite electrode comprising:
- a plurality of platinum nanoparticles in a nanocomposite ink;
- a plurality of multiwall carbon nanotubes in the nanocomposite ink;
- and
- a conductive polymer in the nanocomposite ink, on a flexible substrate, wherein a greater amount of the conductive polymer is present in the nanocomposite ink than an amount of either or both of the plurality of platinum nanoparticles or the plurality of multiwall carbon nanotubes in the nanocomposite ink such that the conductive polymer forms a binder, while also decreasing tunneling distance,
- wherein said nanocomposite electrode comprises glutamate oxidase on its surface and detects L-glutamate via direct electron transfer in response signal to an applied potential.
2 more elements
A method of detecting L-glutamate in a subject, comprising:
- providing a biosensor, wherein said biosensor comprises a nanocomposite electrode comprising:
- a plurality of platinum nanoparticles in a nanocomposite ink;
- a plurality of multiwall carbon nanotubes in the nanocomposite ink;
- and
- a conductive polymer in the nanocomposite ink, on a flexible substrate, wherein a greater amount of the conductive polymer is present in the nanocomposite ink than an amount of either or both of the plurality of platinum nanoparticles or the plurality of multiwall carbon nanotubes in the nanocomposite ink such that the conductive polymer forms a binder while also decreasing tunneling distance,
- wherein said nanocomposite electrode comprises glutamate oxidase on its surface;
- applying a potential to the biosensor;
- reading an amperometric response signal generated from direct electron transfer on the nanocomposite electrode surface in response to the applied potential;
- and
- detecting L-glutamate in the subject based on the amperometric response signal.
7 more elements
Dependencies are inferred from the claim text.