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US 12,704,519 B2Granted
Methods for detecting assay interferents and increasing dynamic range
AssigneeAbbott Laboratories
InventorsMark R. Pope, Stefan J. Hershberger, Qiaoqiao Ruan, Kerry M. Swift, Sergey Y. Tetin, Patrick Macdonald, Richard A. Haack
Life of this patent
Estimating the term…
Abstract
The disclosure provides kits and methods for detecting a substance that interferes with detection of an analyte in a sample and for expanding the dynamic range and reducing the hook effect of an immunoassay. The kits and methods employ two conjugates with two different detectable labels, at least one of which is a chemiluminescent compound of Formula (I).
Claims26
A kit comprising:
- (a) a first conjugate comprising a first detectable label attached to a first specific binding member that specifically binds an analyte,
- (b) a second conjugate comprising a second detectable label attached to a second specific binding member, wherein the second specific binding member specifically binds a substance which interferes with detection of the analyte in a sample,
- (c) a third specific binding member attached to a solid support, which either concurrently or competitively binds to the analyte and the substance which interferes with detection of the analyte, and optionally
- (d) a fourth specific binding member attached to the solid support, which specifically binds to the substance which interferes with detection of the analyte, wherein at least one of the first and second detectable labels comprises a compound of formula (I), or a salt thereof:
- wherein:
- X is ethylenediamine, 4,7,10-trioxa-1,13-tridecanediamine,
- Y is nitrogen, oxygen, or sulfur,
- wherein when Y is nitrogen, R1 is —SO2-A, wherein A is phenyl that is unsubstituted or substituted with 1 or 2 substituents selected from C1-C4 alkyl, and when Y is oxygen or sulfur, R1 is absent;
- Q is —SO2— or —CO—;
- L1 and L2 are each independently a C1-C4 alkylene;
- R2 is —COOZ;
- Z is hydrogen or C1-C4 alkyl;
- and
- Ra, Rb, Rc, Rd, Re, Rf, Rg, and Rh are each hydrogen.
11 more elements
A kit comprising:
- (a) a first specific binding member comprising a biotin molecule and a first detectable label attached thereto, wherein the first specific binding member specifically binds an analyte;
- (b) a conjugate comprising a second detectable label attached to a second specific binding member that specifically binds to the analyte;
- (c) a solid support coated with streptavidin, wherein the streptavidin binds to the biotin molecule attached to the first specific binding member and a biotin molecule which interferes with detection of the analyte in a sample,
- wherein at least one of the first and second detectable labels comprises a compound of formula (I), or a salt thereof:
- wherein:
- X is ethylenediamine, 4,7,10-trioxa-1,13-tridecanediamine,
- Y is nitrogen, oxygen, or sulfur,
- wherein when Y is nitrogen, R1 is —SO2-A, wherein A is phenyl that is unsubstituted or substituted with 1 or 2 substituents selected from C1-C4 alkyl, and when Y is oxygen or sulfur, R1 is absent;
- Q is —SO2— or —CO—;
- L1 and L2 are each independently a C1-C4 alkylene;
- R2 is —COOZ;
- Z is hydrogen or C1-C4 alkyl;
- and
- Ra, Rb, Rc, Rd, Re, Rf, Rg, and Rh are each hydrogen.
11 more elements
A kit comprising:
- (a) a first conjugate comprising a first detectable label attached to a first specific binding member that binds an analyte,
- (b) a second conjugate comprising a second detectable label attached to a second specific binding member, wherein the second specific binding member binds the same analyte as the first specific binding member and the binding affinity of the first specific binding member for the analyte is greater than that of the second specific binding member, and
- (c) a third specific binding member attached to a solid support, which can bind to the analyte concurrently with either the first or second specific binding member, wherein at least one of the first and second detectable labels comprises a compound of formula (I), or a salt thereof:
- wherein:
- X is ethylenediamine, 4,7,10-trioxa-1,13-tridecanediamine,
- Y is nitrogen, oxygen, or sulfur,
- wherein when Y is nitrogen, R1 is —SO2-A, wherein A is phenyl that is unsubstituted or substituted with 1 or 2 substituents selected from C1-C4 alkyl, and when Y is oxygen or sulfur, R1 is absent;
- Q is —SO2— or —CO—;
- L1 and L2 are each independently a C1-C4 alkylene;
- R2 is —COOZ;
- Z is hydrogen or C1-C4 alkyl;
- and
- Ra, Rb, Rc, Rd, Re, Rf, Rg, and Rh are each hydrogen.
10 more elements
A kit comprising:
- (a) a first conjugate comprising a first specific binding member that binds an analyte and a first detectable label;
- (b) a second conjugate comprising a second specific binding member and a second detectable label, wherein (i) the first specific binding member and second specific binding member are the same or are different, and (ii) the first and second detectable labels are different;
- (c) a third specific binding member attached to a solid support, which can bind to the analyte concurrently with either the first or second specific binding member, wherein at least one of the first and second detectable labels comprises a compound of formula (I), or a salt thereof:
- wherein:
- X is ethylenediamine, 4,7,10-trioxa-1,13-tridecanediamine,
- Y is nitrogen, oxygen, or sulfur,
- wherein when Y is nitrogen, R1 is —SO2-A, wherein A is phenyl that is unsubstituted or substituted with 1 or 2 substituents selected from C1-C4 alkyl, and when Y is oxygen or sulfur, R1 is absent;
- Q is —SO2— or —CO—;
- L1 and L2 are each independently a C1-C4 alkylene;
- R2 is —COOZ;
- Z is hydrogen or C1-C4 alkyl;
- and
- Ra, Rb, Rc, Rd, Re, Rf, Rg, and Rh are each hydrogen.
10 more elements
Dependencies are inferred from the claim text.