Engineered binding proteins for recognition of bacteria
AssigneeMassachusetts Institute of Technology
InventorsHadley Sikes Johnson, Ki-Joo Sung, Yining Hao
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Abstract
Described herein are antigen-binding molecules that bind to bacteria (e.g., Listeria monocytogenes) and methods of use thereof. Also described herein are compositions and kits comprising these antigen-binding molecules.
Claims18
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein,
- wherein the antigen-binding molecule comprises a reduced charge Sso7d (rcSso7d) antigen-binding protein that binds:
- a) Listeria monocytogenes protein LMOf2365_0639, wherein the antigen-binding protein comprises a variable region having the amino acid sequence of any one of SEQ ID NOs:
- 1-8;
- or
- b) Salmonella typhi Native Omp, said Native Omp comprising the amino acid sequence of Salmonella typhi membrane proteins OmpA, OmpW, OmpC, OmpF, OmpD, and phoE, wherein the antigen-binding protein comprises a variable region having the amino acid sequence of any one of SEQ ID NOs:
- 58-71;
- or
- c) Escherichia coli OmpW, wherein the antigen-binding protein comprises a variable region having the amino acid sequence of any one of SEQ ID NOs:
- 73-80;
- or
- d) Escherichia coli OmpA, wherein the antigen-binding protein comprises a variable region having the amino acid sequence of any one of SEQ ID NOs:
- 81-83.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein, wherein the rcSso7d antigen-binding protein comprises the amino acid sequence of any one of SEQ ID NOs:
- 13-19 or 33-57.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein, wherein the rcSso7d antigen-binding protein comprises the amino acid sequence of any one of SEQ ID NOs:
- 13-19 or 33-57 or an amino acid sequence having no more than seven conserved amino acid substitutions with any one of SEQ ID NOs:
- 55-57, wherein any conserved amino acid substitutions do not occur in a variable region of the rcSso7d antigen-binding protein.
Dependencies are inferred from the claim text.