Signal amplification in plasmonic specific-binding partner assays
AssigneeZoetis Services LLC
InventorsRajesh K. Mehra, Vincent Chiang, Kenneth P. Aron, Asher Krell
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Abstract
The present invention relates to analyte detection devices and methods of using such devices to detect minute quantities of a target analyte in a sample. In particular, the invention provides an analyte detection device comprising a plurality of composite metallic nanostructures conjugated to analyte binding partners and a surface containing a metallic nanolayer on which a plurality of capture molecules is immobilized. Methods of preparing composite nanostructures are also described.
Claims31
A localized surface plasmon resonance (LSPR) analyte detection device comprising:
- a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to binding partners that are capable of specifically binding to a target analyte, wherein the composite metallic nanostructures comprise a silver core and a gold coating;
- a surface containing a metallic nanolayer;
- and
- a plurality of capture molecules, wherein the capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analyte.
A localized surface plasmon resonance (LSPR) analyte detection device comprising:
- a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to target analytes, wherein the composite metallic nanostructures comprise a silver core and a gold coating;
- a surface containing a metallic nanolayer;
- and
- a plurality of capture molecules, wherein the capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analytes.
A method of detecting a target analyte in a sample based on localized surface plasmon resonance (LSPR), the method comprising:
- mixing the sample with a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to binding partners that are capable of specifically binding to the target analyte if present in the sample to form analyte-detection conjugate complexes, wherein the composite metallic nanostructures comprise a silver core and a gold coating;
- contacting the mixture with a surface containing a metallic nanolayer, wherein a plurality of capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analyte if present in the sample;
- exposing the surface to a light source at a wavelength range within the ultraviolet-visible-infrared spectrum;
- and
- measuring an optical signal from the surface, wherein a change in the optical signal indicates the presence of the target analyte in the sample.
Dependencies are inferred from the claim text.