Methods and kits for detecting target substances
AssigneeJl Medilabs, Inc
InventorJong Jin Lee
Life of this patent
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Abstract
Provided is a method for detecting target substances. The method includes a) introducing a sample containing target substances onto a substrate, b) allowing detection probes conjugated with docking strands to specifically bind to the target substances, c) introducing one or more separate strands capable of complementary binding to the docking strands into the docking strands, either the docking strands or the separate strands, or both, are labeled with at least one donor fluorescent substance and at least one acceptor fluorescent substance, and d) measuring fluorescence signals generated by the FRET between the donor fluorescent substance and the acceptor fluorescent substance to identify the target substances.
Claims20
A method for detecting target substances, comprising:
- a) introducing a sample containing target substances onto a substrate;
- b) allowing detection probes conjugated with docking strands to specifically bind to the target substances;
- c) introducing separate strands capable of complementary binding to the docking strand,
- wherein the separate strands comprise one or more of donor strand labeled with a first donor fluorescent substance and one or more of acceptor strand labeled with a second donor fluorescent substance and an acceptor fluorescent substance that forms a FRET pair with the second donor fluorescent substance;
- and
- d) measuring fluorescence signals generated by the FRET between the second donor fluorescent substance and the acceptor fluorescent substance to identify the target substances.
Dependencies are inferred from the claim text.