Derivatization of beta-lactam antibiotics as calibrators/ISTD in massspec measurements
AssigneeRoche Diagnostics Operations, Inc.
InventorsFlorian Huber, Thomas Scheidt, Gaston Hubertus Maria Vondenhoff
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Abstract
The invention relates to a complex comprising an antibiotic substance and a nucleophilic derivatization reagent, compositions comprising the complex, kits comprising complex or composition, as well as uses of the complex or composition.
Claims17
A complex formed of an antibiotic substance and a nucleophilic derivatization reagent, the complex including an isotopic label, wherein the nucleophilic derivatization reagent is a linear primary amine comprising a primary amine group and in the range of from 3 to 10 C-atoms, and wherein the antibiotic substance is a beta-lactam antibiotic.
A derivatized beta-lactam antibiotic substance comprising an isotopic label, derivatized with a nucleophilic derivatization reagent, wherein the nucleophilic derivatization reagent is a linear primary amine comprising a primary amine group and in the range of from 3 to 10 C-atoms, the derivatized beta-lactam antibiotic substance being suitable as an internal standard (ISTD) and/or a calibrator in Mass Spectrometry Measurements.
A method of producing a stabilized antibiotic substance, comprising derivatizing a beta-lactam antibiotic substance with a nucleophilic derivatization reagent to stabilize the antibiotic substance, wherein the nucleophilic derivatization reagent is a linear primary amine comprising a primary amine group and in the range of from 3 to 10 C-atoms, wherein the antibiotic substance and/or the nucleophilic derivatization reagent comprises one or more isotopic label.
Dependencies are inferred from the claim text.