Stimulated Raman scattering tomography system and method
AssigneeNational University of Singapore
InventorsZhiwei Huang, Li Gong, Shulang Lin
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Abstract
A stimulated Raman scattering tomography system includes means for generating a first input light beam, means for generating a second input light beam, an objective, a condenser and a detector. The first input light beam is phase-modulated while the second input light beam is amplitude-modulated. The objective is configured to direct the first and second input light beams onto a sample. The condenser is configured to collect an output light beam from the sample. The is detector configured to detect at least a portion of the output light beam corresponding to the first input light beam. The system further includes means for forming a depth-resolved image of the sample from the detected portion of the output light beam.
Claims15
A stimulated Raman scattering tomography system comprising:
- a laser light source configured to provide a first output and a second output,
- wherein the first output is configured to generate a first input light beam,
- wherein the first input light beam is phase-modulated;
- wherein the second output is configured to generate a second input light beam, wherein the second input light beam is amplitude-modulated;
- and
- wherein the first input light beam and the second input light beam are configured to be form collinear Bessel beams, the collinear Bessel beams configured to generate an axial optical beating pattern comprising a plurality of axial spatial-frequency components along an axial direction;
- an objective configured to direct the collinear Bessel beams onto a sample, wherein depth information of the sample is encoded into the plurality of axial spatial-frequency components of the optical beating pattern;
- a condenser configured to collect an output light beam from the sample;
- a detector configured to detect at least a portion of the output light beam corresponding to the first input light beam;
- and
- a lock-in amplifier configured to demodulate the detected portion of the output light beam from the sample for forming a depth-resolved image of the sample from the detected portion of the output light beam, wherein the depth-resolved image is formed based on the encoded axial spatial-frequency components without scanning a focal spot of the first or second input light beam across a depth direction of the sample.
A stimulated Raman scattering tomography method comprising:
- generating a first input light beam, wherein the first input light beam is phase-modulated;
- generating a second input light beam, wherein the second input light beam is amplitude-modulated;
- forming the first input light beam and the second input light beam into collinear Bessel beams, the collinear Bessel beams configured to generate an axial optical beating pattern comprising a plurality of axial spatial-frequency components along an axial direction;
- directing the collinear Bessel onto a sample, wherein depth information of the sample is encoded into the plurality of axial spatial-frequency components of the optical beating pattern;
- collecting an output light beam from the sample;
- detecting at least a portion of the output light beam corresponding to the first input light beam;
- and
- forming a depth-resolved image of the sample from the detected portion of the output light beam, wherein the depth-resolved image is formed based on the encoded axial spatial-frequency components without scanning a focal spot of the first or second input light beam across a depth direction of the sample.
Dependencies are inferred from the claim text.