Filter set, fluorescence observation system and method for simultaneously observing fluorescent and non-fluorescent regions of an object

Fluorescence observation system for simultaneously observing fluorescent and non-fluorescent regions of an object, comprising:

• an illumination system having a light source for illuminating the object;
• an observation system for imaging the object;
• and
• a filter set comprising an illumination filter and an observation filter,
• wherein the average value of TI(λ) over a first wavelength range from a wavelength λVIS,MAX up to a wavelength λ1 is greater than a first value W1;
• wherein the average value of TI(λ) over a second wavelength range from a wavelength λ2 up to a wavelength λVIS,MAX is smaller than a second value W2;
• wherein the average value of TO(λ) over the first wavelength range is smaller than the second value W2;
• wherein the average value of TO(λ) over the second wavelength range is greater than the first value W1;
• wherein the average value of
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• over a third wavelength range, which is a union of the first and the second wavelength ranges, is smaller than a third value W3 so that the object is observable with color fidelity, wherein μWLB3 is the average value of TI(λ)·TO(λ) over the third wavelength range;
• wherein TI(λ) is the wavelength-dependent transmittance of the illumination filter,
• wherein TO(λ) is the wavelength-dependent transmittance of the observation filter;
• wherein λVIS,MIN<λ1<λ2<λVIS,MAX, λVIS,MIN=380 nm and λVIS,MAX=780 nm;
• wherein W1>100·W2;
• wherein W3<1.5;
• wherein μWLB3>K1·μWLB4 with K1=10, wherein μWLB4 is the average value of TI(λ)·TO(λ) over a fourth wavelength range from the wavelength λ1 up to the wavelength λ2;
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• wherein the illumination filter of the filter set is arranged in an illumination beam path between the light source and the object and the observation filter of the filter set is arranged in an observation beam path of the observation system.

Method for simultaneously observing fluorescent and non-fluorescent regions of an object using a filter set, wherein the method comprises:

• filtering an illumination light beam, which is directed onto the object, using an illumination filter of the filter set;
• and
• filtering light emanating from the object using an observation filter of the filter set,
• wherein the average value of TI(λ) over a first wavelength range from a wavelength λVIS,MIN up to a wavelength λ1 is greater than a first value W1;
• wherein the average value of TI(λ) over a second wavelength range from a wavelength λ2 up to a wavelength λVIS,MAX is smaller than a second value W2;
• wherein the average value of TO(λ) over the first wavelength range is smaller than the second value W2;
• wherein the average value of TO(λ) over the second wavelength range is greater than the first value W1;
• wherein the average value of
• ❘
• "\[LeftBracketingBar]"
• log
• 10
• (
• T
• I
• (
• λ
• )
• ·
• T
• O
• (
• λ
• )
• μ
• WLB
• ⁢
• 3
• )
• ❘
• "\[RightBracketingBar]"
• over a third wavelength range, which is a union of the first and the second wavelength ranges, is smaller than a third value W3 so that the object is observable with color fidelity, wherein μWLB3 is the average value of TI(λ)·TO(λ) over the third wavelength range;
• wherein TI(λ) is the wavelength-dependent transmittance of the illumination filter,
• wherein TO(λ) is the wavelength-dependent transmittance of the observation filter;
• wherein λVIS,MIN<λ1<λ2<λVIS,MAX, λVIS,MIN=380 nm and λVIS,MAX=780 nm;
• wherein W1>100·W2;
• wherein W3<1.5;
• and
• wherein μWLB3>K1·μWLB4 with K1=10, wherein μWLB4 is the average value of TI(λ)·TO(λ) over a fourth wavelength range from the wavelength λ1 up to the wavelength λ2.