Systems and methods for identification of MHC-I peptide epitopes

A method of purifying a peptide of interest that binds an MHC class I molecule, the method comprising:

• contacting a precursor peptide-MHC-I complex (p*MHC-I) comprising a MHC Class I heavy chain, a β2 microglobulin, and a precursor peptide with a molecular chaperone, thereby forming a peptide receptive MHC-I complex comprising the MHC class I heavy chain and the β2 microglobulin;
• affixing the peptide receptive MHC-I complex to a solid substrate;
• contacting the peptide receptive MHC-I complex with a plurality of peptides of interest, wherein at least one of the plurality of peptides of interest binds the peptide-receptive MHC-I complex, resulting in the formation of a peptide-MHC-I complex (pMHC-I);
• and
• removing the pMHC-I from the solid substrate, thereby purifying the peptide of interest.