Method for labeling nucleic acid
AssigneeCambridge Enterprise Limited
InventorsManuel Nappi, Alexandre Hofer, Shankar Balasubramanian, Matthew J. Gaunt
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Abstract
The invention provides a method for labeling a nucleic acid comprising N6-methyl adenine. The method comprises forming an alpha-amino radical on the N6-methyl group of N6mAde, and capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group. The presence of N6mAde in a nucleic acid may then be established by detection of the labeled nucleic acid, or the labeled nucleic acid may be extracted or further modified using the label. Also provided is a method for mapping the position of N6mAde within a target nucleic acid, and probe molecules and kits for use in the method.
Claims16
A method for labeling a nucleic acid comprising N6-methyl adenine (N6mAde), the method comprising:
- i) forming an alpha-amino radical on the N6-methyl group of N6mAde;
- and
- ii) capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group (O═N-Py-).
Dependencies are inferred from the claim text.