Methods and systems to secrete lignin-modifying enzymes and uses thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorNikita A. Khlystov
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Abstract
Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, yeast strains, including S. cerevisiae, are used to produce and secrete lignin-modifying enzymes Further embodiments are directed to methods to screening peroxidase-producing yeast strains, including S. cerevisiae. Additional embodiments are directed to an expression vector or cassette encoding for a protein of interest and one or more proteins to allow for surface to display of the protein of interest.
Claims5
A transgenic yeast organism comprising:
- a knockout of a first native gene and a knockout of a second native gene, wherein the first native gene is cyt2 encoding cytochrome c heme lyase and the second native gene is pmt2 encoding protein O-mannosyltransferase 2;
- and
- an expression cassette comprising a nucleotide sequence encoding for a lignin-modifying enzyme.
Dependencies are inferred from the claim text.