Composition and methods for producing tobacco plants and products having increased phenylalanine and reduced tobacco-specific nitrosamines (TSNAs)
AssigneeAltria Client Services LLC
InventorsChengalrayan Kudithipudi, Dongmei Xu, James Strickland, Yanxin Shen
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Abstract
The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), increase phenylalanine, or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.
Claims10
A cured tobacco leaf of a modified tobacco plant, wherein said cured tobacco leaf comprises a decreased amount of one or more tobacco specific nitrosamines (TSNAs), a recombinant nucleic acid molecule comprising a promoter operably linked to a first polynucleotide encoding a Chorismate Mutase-like polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
- 68 to 70, and a recombinant nucleic acid molecule comprising a promoter operably linked to a second polynucleotide encoding a bHLH-like transcription factor polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
- 5 and 6, wherein said decreased amount is compared to an unmodified control tobacco plant.
Dependencies are inferred from the claim text.