Engineered microorganisms

An engineered bacterium comprising one or more gene(s) or gene cassette(s) encoding a therapeutic molecule and a modified pks island as compared to a corresponding bacterium comprising an unmodified pks island,

• wherein the modified pks island comprises
• 1) a full or partial deletion of the clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, and clbR gene sequences;
• and
• 2) an intact or unmodified clbS gene sequence;
• and
• 3) a full or partial deletion of the promoter operably linked to clbS;
• wherein the therapeutic molecule is a protein, and
• wherein the engineered bacterium is an engineered Escherichia coli.