Subtilase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsFrank Winther Rasmussen, Peter Kamp Hansen, Lars Lehmann Hylling Christensen
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Abstract
The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
Claims21
A subtilase variant having protease activity, wherein the amino acid sequence of the variant has at least 80% but less than 100% sequence identity to SEQ ID NO:
- 1, and wherein the variant comprises the substitution N76D and one or more additional substitutions selected from N185{C,E}, S188{C,D,E}, and Q191N, wherein each position corresponds to the position of the polypeptide of SEQ ID NO:
- 2.
A method for producing a subtilase variant, comprising
- (a) introducing into a parent subtilase the substitution N76D and one or more additional substitutions selected from N185{C,E}, S188{C,D,E}, and Q191N, wherein each position corresponds to the position of the polypeptide of SEQ ID NO:
- 2, and wherein the amino acid sequence of the variant has at least 80% but less than 100% sequence identity to SEQ ID NO:
- 1, and
- (b) recovering the variant.
Dependencies are inferred from the claim text.