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AssigneeBRL Medicine (Shanghai) Co., Ltd.
InventorsJiqin Zhang, Jiaxuan Yang, Yue Tian, Bing Du, Dali Li, Mingyao Liu, Zaixi Xi
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Disclosed is an sgRNA guiding a PD1 gene for cleavage to achieve the efficient integration of exogenous sequences. The method for gene editing a PD1 gene in cells includes the steps of introducing a nuclease and an sgRNA into cells, and gene-editing the PD1 gene. The sgRNA guides the nuclease to cleave the PD1 gene and forms a broken site, at which an exogenous donor repair template can also be introduced, so that CAR-T elements can be directionally inserted at the specific site of the PD1 locus to construct enhanced CD19-CART cells with PD1 knockout in one step.
A method for gene editing a PD1 gene in cells, comprising the steps of introducing a nuclease and an sgRNA into the cells, and gene-editing the PD1 gene, wherein the sgRNA guides the nuclease to cleave the PD1 gene and forms a broken site,
A method for gene editing a PD1 gene in cells, comprising the steps of introducing a nuclease and an sgRNA into the cells, and gene-editing the PD1 gene, wherein the sgRNA guides the nuclease to cleave the PD1 gene and forms a broken site, wherein the method comprises the steps of providing and introducing a donor repair template into the broken site of the cells in a non-viral manner;
Dependencies are inferred from the claim text.