Stem cell and tissue cultures and their products

A method of cell culturing, said method comprising the following steps in order:

• a) obtaining an umbilical cord tissue;
• b) cleaning said umbilical cord tissue with a sterilizing agent;
• c) mechanically dissociating said umbilical cord tissue into fragments;
• d) liberating a cell mixture from said fragments to form a decellularized tissue matrix, wherein said cell mixture includes differentiated cells, stem cells and progenitor cells;
• e) culturing i) said decellularized tissue matrix, or ii) said cell mixture, or both i) and ii) with serum, plasma or platelet lysate to form a combination, wherein said serum, plasma or platelet lysate is autologous or syngeneic or HLA-matched to at least 3 matched MHC loci and optionally gender-matched to said umbilical cord tissue;
• f) culturing said combination under hypoxic conditions;
• g) adding stem cells to said combination, wherein said stem cells are obtained from a biological source that is autologous or syngeneic or HLA matched at a same at least 3 matched MHC loci to said umbilical cord tissue;
• and
• h) further culturing said combination under hypoxic conditions to amplify said stem cells.