In vitro method of differentiating a human pluripotent stem cell population into a cardiomyocyte cell population
AssigneeNcardia B.V.
InventorsStefan Robbert Braam, Ana Catarina Martins Grandela
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Abstract
The current invention relates to a method of differentiation of human pluripotent stem cells into a human stem-cell derived population of cardiomyocytes. The method comprises the use of specific combination of steps and compounds to induce and/or promote differentiation. The method also comprises steps directed to further maturation of the cardiomyocytes obtained with the method of the invention. Also provided are kits for use in a method of differentiation as well as cell populations obtainable with the method disclosed.
Claims3
A composition comprising a pluripotent stem cell (PSC) population in an aqueous media with a first compound that stimulates betacatenin degradation and a second compound that suppresses phosphorylation of dishevelled 2, wherein the first compound is a tankyrase inhibitor that comprises XAV-939 and the second compound is a porcupine inhibitor that comprises IWP-L6, and wherein the XAV-939 is in a concentration of from 1 μM to about 10 μM and the IWP-L6 is in a concentration of from 0.25 μM to about 2 μM.
Dependencies are inferred from the claim text.