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US 12,703,846 B2Granted
Apparatus and a method for continuously harvesting a biological substance produced by a cultured cell
AssigneeWuxi Biologics Ireland Limited
InventorsWeichang Zhou, Hang Zhou, Mingyue Fang
Life of this patent
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Abstract
An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell. An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell using 2 sets of filters having different pore sizes.
Claims23
An apparatus for continuously harvesting a biological substance produced by cultured cells, wherein the apparatus comprises:
- (a) filter 1 which is at least one filter(s) having pore size(s) which do(es) not allow the cells to pass through the filter, but allow the biological substance to pass through the filter,
- (b) filter 2 which is at least one filter(s) having pore size(s) which do(es) not allow both the cells and the biological substance to pass through the filter, but keep fluid-flow-through of the filter,
- (c) a regulator that regulates the filtration rate of filter 1, and
- (d) a regulator that regulates the filtration rate of filter 2
- wherein filter 1 and filter 2 are fluidly connected to an apparatus for culturing the cells in a parallel manner and
- wherein the (c) and (d) regulate the ratio of flow rate of cell culture through filter 1 and filter 2 to concentrate the biological substance first and then do not concentrate the biological substance in harvest liquid.
3 more elements
A method for continuously harvesting a biological substance produced by cultured cells, comprising:
- continuously harvesting a biological substance produced by cells cultured in an apparatus for culturing the cells with a basal medium and a feed medium in a perfusion manner with an apparatus comprising:
- (a) filter 1 which is at least one filter(s) having pore size(s) which do(es) not allow the cells to pass through the filter, but allow the biological substance to pass through the filter, and
- (b) filter 2 which is at least one filter(s) having pore size(s) which do(es) not allow both the cells and the biological substance to pass through the filter, but keep fluid-flow-through of the filter;
- and
- (c) a regulator that regulates the filtration rate of filter 1, and
- (d) a regulator that regulates the filtration rate of filter 2,
- wherein filter 1 and filter 2 are fluidly connected to the apparatus for culturing the cells in a parallel manner, and
- wherein the (c) and (d) regulate the ratio of flow rate of cell culture through filter 1 and filter 2 to concentrate the biological substance first and then do not concentrate the biological substance in harvest liquid.
5 more elements
A method for producing a biological substance, comprising:
- culturing cells in an apparatus for culturing the cells with a basal medium and a feed medium in a perfusion manner;
- and
- continuously harvesting a biological substance produced by the cultured cells with an apparatus comprising:
- (a) filter 1 which is at least one filter(s) having pore size(s) which do(es) not allow the cells to pass through the filter, but allow the biological substance to pass through the filter, and
- (b) filter 2 which is at least one filter(s) having pore size(s) which do(es) not allow both the cells and the biological substance to pass through the filter, but keep fluid-flow-through of the filter;
- and
- (c) a regulator that regulates the filtration rate of filter 1, and
- (d) a regulator that regulates the filtration rate of filter 2,
- wherein filter 1 and filter 2 are fluidly connected to the apparatus for culturing the cells in a parallel manner, and
- wherein the (c) and (d) regulate the ratio of flow rate of cell culture through filter 1 and filter 2 to concentrate the biological substance first and then do not concentrate the biological substance in harvest liquid.
7 more elements
Dependencies are inferred from the claim text.