Humanized antibody targeting the tumor associated antigen IL13Ra2
AssigneeNorthwestern University
InventorIrina V. Balyasnikova
Life of this patent
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Abstract
The present invention provides humanized antibodies that bind to IL13Rα2, an interleukin-13 receptor that is overexpressed by the majority of glioblastoma tumors and not expressed at significant levels in normal brain tissue. Also provided are bispecific T cell engagers that bind to both IL13Rα2 and to the T cell co-receptor CD3 as well as methods for treating cancer, in which these humanized antibodies are used to target tumors.
Claims20
A humanized antibody that binds to IL13Rα2 comprising:
- a variable light domain (VL) comprising an amino acid sequence of SEQ ID NO:53;
- and
- a variable heavy domain (VH) comprising an amino acid sequence of SEQ ID NO:54.
A humanized antibody that binds to IL13Rα2, comprising:
- (a) a heavy chain comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, or SEQ ID NO:12;
- and
- (b) a light chain comprising SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:
- 7, SEQ ID NO:8, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or SEQ ID NO:16.
An engineered bispecific antibody comprising a first single-chain variable fragment (scFv) that binds to CD3 and a second scFv that binds to IL13Rα2, wherein the first scFv comprises:
- (a) a variable light domain (VH) comprising an amino acid sequence of SEQ ID NO:51;
- a
- (b) a first flexible linker;
- and
- (c) a variable heavy domain (VL) comprising an amino acid sequence of SEQ ID NO:52, and
- wherein the second scFv comprises:
- (d) a variable light domain (VL) comprising an amino acid sequence of SEQ ID NO:53;
- (e) a second linker;
- and
- (f) a variable heavy domain (VH) comprising an amino acid sequence of SEQ ID NO:54;
- and
- wherein the bispecific antibody comprises from N terminal to C terminal:
- the VH of the first scFv, the VL of the first scFv, the VL of the second scFv, and the VH of the second scFv.
Dependencies are inferred from the claim text.