Recombinant mucins, and compositions and methods for using the same
AssigneeCornell University
InventorsMatthew Paszek, Heidi Reesink, Carolyn Shurer, Hao Pan
Life of this patent
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Abstract
Provided are compositions and methods related to improved mucins, methods of making the improved mucins, and cells and cell cultures that express glycosylated mucins. The compositions and methods provide improved cell cultures, and improved methods of producing co-expressed proteins that are distinct from the mucins.
Claims14
Modified mammalian cells present in a suspension cell culture, said culture being present in a suspended cell bioreactor, the modified mammalian cells comprising polypeptides expressed from recombinant polynucleotides introduced into the cells, wherein the polypeptides comprise a transmembrane anchor and a segment external to the cells, the segment external to the cells comprising a repeated amino acid sequence consisting of the sequence PDTRPAPGSTAPPAHGVTSA (SEQ ID NO:
- 8), said sequence being repeated only 21 or 42 times, and wherein the cells in the suspension cell culture exhibit less aggregation relative to a control value obtained from a suspended cell culture comprising mammalian cells that do not express the polypeptide comprising the repeated amino acid sequence.
Dependencies are inferred from the claim text.