Poptag peptide and uses thereof
AssigneesCZ Biohub SF, LLC, The Board of Trustees of the Leland Stanford Junior University
InventorsKeren Lasker, Steven Boeynaems, Aaron David Gitler, Lucy Shapiro
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Abstract
Proteins and fusion proteins for forming membraneless droplets in cells are provided. Described herein, is the development of a protein, named PopTag, that drives phase separation when it is part of a chimeric fusion protein. PopTag is engineered from the PopZ protein, found in a-proteobacteria (including Caulobacter crescentus). Despite PopZ being exclusively found in this clade of bacteria, the PopTag can drive protein phase separation in other prokaryotes (e.g., E. coli) and eukaryotes (e.g., human cells).
Claims21
A fusion protein comprising an amino acid sequence linked to a polypeptide sequence comprising SEQ ID NO:
- 1, or a variant thereof as set forth in Table 1, wherein the amino acid sequence is heterologous to the polypeptide sequence.
Dependencies are inferred from the claim text.