System and methods for scalable melanin purification
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsAnna M Crumbley, Frank J Kragl, Phuong Vi Luu Le, Henry S Gibbons
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Abstract
Provided are systems and methods for isolating melanins from a medium that include providing tangential filtration of the medium across a membrane with a 300 kilo Dalton or less cutoff. In some aspects, the methods and systems are suitable for large scale preparations due to the inherent established scalability of the unit operations employed herein. The systems and the methods can be utilized to purify melanins from fermented cell medium as well as from lysates generated therefrom.
Claims12
A method to isolate one or more melanins from an aqueous medium, the method comprising:
- providing the aqueous medium comprising the one or more melanins therein to an inlet of a tangential flow filtration (TFF) enclosure at a positive pressure, wherein the TFF enclosure comprises a retentate outlet and a permeate outlet;
- and
- flowing the aqueous medium across a first membrane within the TFF enclosure toward the retentate outlet, wherein the membrane has a size restriction of about 300 kilo Daltons (kDa) or less to isolate the one or more melanins from the aqueous medium, and wherein the permeate outlet is operably connected to a permeate storage container for collecting permeate.
Dependencies are inferred from the claim text.