Methods and compositions related to lanthanide-encoded microbeads
AssigneesCZ Biohub SF, LLC, The Board of Trustees of the Leland Stanford Junior University, University of Copenhagen
InventorsYinnian Feng, Adam K. White, Jamin B. Hein, Polly M. Fordyce
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Abstract
The present disclosure provides methods, devices, systems and kits for producing polymeric microbeads, including lanthanide-encoded microbeads. Among others, the present disclosure provides methods, systems and kits for producing functionalized microbeads that include on their surfaces amphipathic moieties with free reactive groups that remain free and can be used for covalently coupling molecules or moieties of inters to the microbeads.
Claims20
A method for producing polymeric microbeads comprising lanthanide nanoparticles, comprising:
- i) providing a first fluid comprising a microbead matrix component and lanthanide nanoparticles, and a second fluid, wherein the first fluid and the second fluid are immiscible;
- ii) contacting the first fluid with the second fluid in a microfluidic device, thereby forming droplets of the first fluid;
- iii) removing the formed droplets from the microfluidic device;
- and
- iv) solidifying the microbead matrix component of the formed droplets after the formed droplets are removed from the microfluidic device, thereby forming the polymeric microbeads comprising lanthanide nanoparticles outside of the microfluidic device.
Dependencies are inferred from the claim text.