Genomic insulator element exhibiting enhancer blocking activities in lymphocytes and uses thereof

A construct comprising at least one copy of a genomic insulator element having a core sequence selected from the group of CACTGCCCTCCAGTGGCCA (SEQ ID NO:1), CAGCGCCACCTGCAGGCCA (SEQ ID NO:2), CTTCCAGCAGGAGGAGGCA (SEQ ID NO:3), TGGCCGCTAGAGGGCACGC (SEQ ID NO:

• 4), AAGCACCATCTACTGGTCT (SEQ ID NO:5), CTGCCGCCAGATGGCGCTC (SEQ ID NO:
• 6), TCAGCACTAGATGGCACCC (SEQ ID NO:7), GAGTGACACCTAGTGGCCC (SEQ ID NO:8), CAGCGCCATCTGGGGGCCG (SEQ ID NO:
• 9), TCGCCAGTAGGGGGCGCAA (SEQ ID NO:10), TGCTGCCCCCTGGTGGCCA (SEQ ID NO:
• 11), TGCTGCTCCCTTATGGCCA (SEQ ID NO:12), AGGCCACCAGATGGCATTG (SEQ ID NO:
• 13), CTGCCACGAGGGGGCGGCA (SEQ ID NO:
• 14), TTGCGCCCCCTGCTGGCGA (SEQ ID NO:15), CGTCGCCACCTTCTGGTAA (SEQ ID NO:
• 16), CAGTGCCCTCTGGTGGTAG (SEQ ID NO:
• 17), TTATGCCCCCTGCAGGACA (SEQ ID NO:18), CGCCCAGAAGGTGGCGGCA (SEQ ID NO:19), and CACTGCCCCCTAGTGGACC (SEQ ID NO:20), wherein the construct is a viral vector, transgene cassette, or transposon system, wherein the genomic insulator element comprises a sequence selected from the group of SEQ ID NO:
• 21, SEQ ID NO:
• 22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:
• 25, SEQ ID NO:
• 26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:
• 30, SEQ ID NO:
• 31, SEQ ID NO:
• 32, SEQ ID NO:
• 33, SEQ ID NO:
• 34, SEQ ID NO:
• 35, SEQ ID NO:
• 36, SEQ ID NO:
• 37, SEQ ID NO:
• 38, SEQ ID NO:39 and SEQ ID NO:
• 40.