Methods of preparing anti-human papillomavirus antigen T cells
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Seervices
InventorsChristian S. Hinrichs, Steven A. Rosenberg
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Abstract
Disclosed are methods of preparing an isolated population of human papillomavirus (HPV)-specific T cells comprise dividing an HPV-positive tumor sample into multiple fragments; separately culturing the multiple fragments; obtaining T cells from the cultured multiple fragments; testing the T cells for specific autologous HPV-positive tumor recognition; selecting the T cells that exhibit specific autologous HPV-positive tumor recognition; and expanding the number of selected T cells to produce a population of HPV-specific T cells for adoptive cell therapy. Related methods of treating or preventing cancer using the T cells are also disclosed.
Claims18
A method of expanding a population of T cells, the method comprising:
- (a) culturing multiple fragments of an HPV+ tumor sample from a cancer patient in the presence of at least one cytokine;
- (b) obtaining T-cells from the cultured fragments;
- (c) expanding the number of T-cells to produce an expanded population of HPV-specific T-cells using one or both of (i) irradiated allogenic feeder cells and (ii) irradiated autologous feeder cells;
- and one or both of (iii) OKT3 antibody and (iv) interleukin-2, wherein the T-cells have not been depleted of CD4+ cells;
- and
- (d) optionally, adding a second culturing step following (c).
Dependencies are inferred from the claim text.