Platelet derivative compositions, and methods of making and using such compositions

A method for restoring hemostasis in a subject,

• wherein said method comprising:
• rehydrating a platelet derivative composition in the form of a powder to form a rehydrated platelet derivative composition;
• and
• administering intravenously an effective amount of platelet derivatives in the rehydrated platelet derivative composition to the subject,
• wherein the platelet derivatives in the rehydrated platelet derivative composition:
• have a CD41 percent positivity of at least 70% when measured using flow cytometry,
• have a phosphatidylserine percent positivity of at least 70%, when measured using Annexin V binding in flow cytometry,
• have a concentration of at least 6,000×103 platelet derivatives/μl,
• are capable of generating thrombin in an in vitro thrombin formation assay, and
• are capable of producing an occlusion by attaining a pressure of 80 kPa in less than 14 minutes when used at a concentration of about 70×103 platelet derivatives/μL in platelet-reduced citrated whole blood in an in vitro total thrombus-formation analysis system (T-TAS), and
• wherein less than 3.5% of the platelet derivatives are microparticles.