Thin, flexible wearable immunosensor for detection of multiple biomarkers/targets in bodily fluids

A layered dressing comprising:

• a permeable wound contact layer for placing in contact with a wound;
• a breathable barrier layer;
• a fluid collection layer disposed between the wound contact layer and breathable barrier layer, the fluid collection layer comprising a biosensor housing portion and a fluid collection portion comprising a plurality of channels each having a terminus at the biosensor housing portion;
• and
• a biosensor sensing array comprising one or more electrodes, the biosensor sensing array being disposed between the biosensor housing portion of the fluid collection layer and the breathable barrier layer,
• wherein:
• the channels within the fluid collection portion of the fluid collection layer are configured to allow the flow of fluid from a wound in only a single direction along the channels, where the channels each comprise a plurality of interconnected half-open saw-tooth-shaped capillary channels;
• and
• the channels comprise a first portion configured to draw fluid from a wound in contact with the wound contact layer, and a second portion proximal to the biosensor sensing array as compared to the first portion, where the width of the channels at the first portion is greater than the width of the channels at the second portion such that, when in use, the channels deliver wound fluid by capillary action from a wound in contact with the wound contact layer to the biosensor sensing array, and where the biosensor sensing array is configured to detect one or more markers in said wound fluid.