Bionic nerve graft and preparation method thereof

A preparation method of a bionic nerve graft which comprises a helical stent, a nanofiber membrane, guide fibers and hBMSC-ECM (human-derived Bone Mesenchymal Stem Cell-Extracellular Matrix), wherein a surface of the helical stent is coated with the nanofiber membrane;

• the guide fibers and the hBMSC-ECM are placed in a tube cavity of the helical stent;
• and the helical stent, the nanofiber membrane and the guide fibers are formed of chitosan;
• wherein the preparation method comprises the following steps:
• providing the chitosan and forming the helical stent by a mold pouring method;
• providing the chitosan and coating the surface of the helical stent with one layer of nanofiber membrane in order to obtain a helical tube with a surface coated with the nanofiber membrane;
• providing the chitosan and forming the guide fibers by a template method;
• and
• filling the helical tube with the surface coated with the nanofiber membrane with the guide fibers and the hBMSC-ECM in order to obtain the bionic nerve graft;
• wherein the step of forming the guide fibers comprises:
• providing a chitosan solution with a concentration of 4 wt % to 7 wt %;
• pouring the chitosan solution into a mold with a groove;
• and
• putting the mold with the chitosan solution into a NaOH solution of 5 to 20% for curing and shaping, and washing with water, drying and demolding in order to obtain the guide fibers.