Methods and applications of protein identification
AssigneeNautilus Subsidiary, Inc.
InventorParag Mallick
Life of this patent
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Abstract
Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.
Claims17
A method of assaying proteins, comprising:
- (a) providing a plurality of full length protein molecules immobilized on a substrate at unique spatial addresses, the unique spatial addresses being immobilized to an individual full length protein molecule of the plurality of full length protein molecules, wherein each unique spatial address is optically resolvable from each other unique spatial address on the substrate;
- (b) binding composite affinity reagents to full length protein molecules of the plurality of full length protein molecules immobilized on the substrate, wherein each individual composite affinity reagent of the composite affinity reagents comprises a linker that is attached to a plurality of antibodies or fragments thereof and a common identifiable tag, and wherein each composite affinity reagent recognizes two or more differing full length the different protein molecules of the plurality of full length protein molecules;
- (c) detecting signals from unique spatial addresses containing the immobilized full length protein molecules that are bound to the composite affinity reagents;
- (d) repeating steps (b) and (c) using different composite affinity reagents;
- and
- (e) detecting a binding pattern comprising presences or absences of signals from each of the different composite affinity reagents bound to the immobilized full length protein molecules at the unique spatial addresses on the substrate.
Dependencies are inferred from the claim text.