Back to results
US 12,699,097 B2Granted
M-protein assays
AssigneeBristol-Myers Squibb Company
InventorsRasa Santockyte, Jianing Zeng, Oscar Puig
Life of this patent
Estimating the term…
Abstract
The disclosure provides methods for measuring M-proteins in a biological sample obtained from a subject, comprising applying purified immunoglobulins to a liquid chromatography (LC) mass spectrometry (MS). In some aspects, the immunoglobulins are purified using an immunocapture (IC). In certain aspects, the subject has a plasma cell disorder, e.g., multiple myeloma.
Claims11
A method of
- measuring M-protein in a biological sample obtained from a subject having a plasma cell disorder, wherein the M-protein is measured by:
- (1) purifying immunoglobulins and free light chains in the biological sample by immunocapture,
- (2) dissociating the purified immunoglobulins, and
- (3) applying the dissociated purified immunoglobulins and free light chains to a liquid chromatography-mass spectrometer (LC-MS);
- wherein the biological sample is a blood sample or urine sample;
- wherein the liquid chromatography (LC) employs (i) a mobile phase A, comprising water with 0.1% formic acid, and (ii) a mobile phase B, comprising 0.1% formic acid in acetonitrile;
- and
- wherein the mass spectrometer (MS) employs a capillary voltage of about 4000 V to about 6000 V.
5 more elements
Dependencies are inferred from the claim text.