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US 12,699,093 B2Granted
Biosensor and method for determination of viruses in coronavirus family
AssigneeMugla Sitki Kocman Universitesi
InventorsUlku Anik, Yudum Tepeli Buyuksunetci
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Abstract
A method and a biosensor for determination of the presence of corona-virus are provided. The method includes qualitative or quantitative measurement of probable corona-virus presence by means of colorimetric and/or electrochemical transducer methods as a result of interaction of S-protein in patient samples by means of a biosensor including ACE2, DPP4 and CD147 bio-receptors as bio-receptor.
Claims10
A method for determining a presence of a corona-virus, comprising:
- qualitatively or quantitatively measuring the presence of the corona-virus in an analyte by an electrochemical transducer method by a biosensor including an ACE2 bio-receptor for a SARS virus and a SARS-2 virus, a DPP4 bio-receptor for a MERS virus, and a CD147 bio-receptor for the SARS-2 virus in a corona-virus family, wherein the method comprises the following process steps:
- contacting the analyte, taken from a person in need, with the bio-receptors immobilized onto a working electrode,
- conducting an interaction between the bio-receptors and S-proteins of the corona-virus existing in the analyte,
- differentiating an impedance on a surface of the working electrode by binding of the S-proteins to the bio-receptors and determining whether the person in need is infected with a corona-virus by measuring a change.
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A method for determining a presence of a corona-virus, comprising:
- quantitatively measuring the presence of the corona-virus in an analyte by a colorimetric transducer method by a biosensor including an ACE2 bio-receptor for a SARS virus and a SARS-2 virus, a DPP-4 bio-receptor for a MERS virus, and a CD147 bio-receptor for the SARS-2 virus in a corona-virus family, wherein the method comprises the following process steps:
- firstly adding a color forming and oxidizing reagent solution into an eppendorf tube to obtain a resulting tube, and afterwards adding a proper amount of the bio-receptors in the resulting tube to obtain a blue reacted bio-receptor solution,
- adding the analyte obtained from a person in need into the blue reacted bio-receptor solution for mixing to lose a blue color as a result, wherein decolorization of the solution indicates the presence of corona-virus in the analyte;
- wherein γ-Fe2O3 magnetic nanoparticles and a 3,3′,5,5′-tetramethylbenzidine (TMB) solution containing H2O2 are used as the color forming and oxidizing reagent solution.
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Dependencies are inferred from the claim text.