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AssigneeInstituto De Investigaciones Agropecuarias
InventorsDaina Grinbergs Salas, Ricardo Javier Chilian, Jaime Humberto Mejias Bassaletti, René Andrés France Iglesias
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An antibody that is used to detect the presence of the Chondrostereum purpureum fungus in a plant sample, where the antibody specifically binds to the endolipogalacturonase enzyme produced by Chondrostereum purpureum (anti-endoPG from now). A kit and a method to detect fungal antigens, particularly, to detect the presence of the silverleaf disease (caused by the Chondrostereum purpureum fungus) in fruit trees, by means of detection of the endoPG enzyme through the binding of said antigen to the antibody, functionalized with gold nanoparticles, where the binding is detected via enzyme-linked immunosorbent assays (ELISA) or an immunochromatographic lateral flow assay.
An antibody to detect presence of Chondrostereum purpureum fungus in a plant sample, wherein the antibody has a heavy chain defined by the amino acid sequence SEQ ID No. 2 and a light chain defined by the amino acid sequence SEQ ID No. 4, and wherein said antibody is conjugated with gold nanoparticles through an L-cysteine residue to form gold nanoparticles+L-cysteine+specific anti-endoPG monoclonal antibody complex (NpsAu+Lc+Ab complex) and specifically binds to enzyme endopolygalacturonase from Chondrostereum purpureum (anti-endoPG antibody), wherein the gold nanoparticles have an average diameter of 25 nm and a surface charge of ~19.9 mV.
Dependencies are inferred from the claim text.