Bacteriolysis method, bacteriolysis aid, and method for determining the presence or absence of bacteria
AssigneeAsahi Kasei Kabushiki Kaisha
InventorYu Ando
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Abstract
A method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid, wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS/cm, whereby bacteriolysis of two or more species of bacteria becomes possible.
Claims39
A method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid,
- wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and
- wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS/cm.
A method for lysing a group of bacteria in a sample, comprising the step of mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid,
- wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and
- wherein the bacteriolysis aid has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.0 to 8.0 mS/cm.
A bacteriolysis aid comprising 0 to 1.5 M of salts, 5 to 500 mM of a buffer, and 2 to 5% of a nonionic surfactant, and having a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.0 to 8.0 mS/cm, in a sample in combination with at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase for lysing a group of bacteria including Escherichia species, Staphylococcus species, and Streptococcus species.
A method for determining the presence or absence of at least one species of bacteria selected from the group consisting of Escherichia species, Staphylococcus species, and Streptococcus species, and Streptococcus species, in a sample, comprising the steps of:
- mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid to prepare a mixture solution;
- and
- determining the presence or absence of the bacteria by detecting a L7/L12 ribosome protein derived from the bacteria contained in the mixture solution by means of an immunological method,
- wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase,
- wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS/cm,
- wherein in the step to prepare the mixture solution, the lysozyme can lyse the Escherichia species, the lysostaphin can lyse the Staphylococcus species, and the lysozyme, acetyl glucosaminidase, and endopeptidase can lyse the Streptococcus species.
Dependencies are inferred from the claim text.