Modified ELISA with hemoglobin correction apparatus and methods thereof

An enzyme-linked immunosorbent bioassay diagnostic kit comprising:

• a fingerstick device;
• and
• a cartridge having a plurality of wells and an optical cuvette, the cartridge having a a pipette tip disposed in one of the plurality of wells and bioassay components disposed in one or more remaining wells of the plurality of wells;
• wherein the pipette tip has sidewalls coated with an antibody;
• and
• wherein the bioassay components comprise at least one reagent being chosen from a group consisting of a pH stabilizer, an ionic catalyst control agent, an ionic intracellular inhibitory control agent, a detergent, a negative control agent, a chelating agent, a biocide preservative, an antibody, a conjugate stabilizing agent, a chromogenic substrate, and a visualizing reagent.

A method of preparing a disposable pipette for use in performing enzyme-linked immunosorbent assays while reducing washing time, the method comprising steps of:

• pretreating a pipette tip by coating an internal surface of the pipette tip, wherein the pretreating step includes:
• disposing a first coating containing an affinity agent along the internal surface of the pipette tip;
• disposing a second coating containing a blocking solution over the first coating along the internal surface of the pipette tip;
• and
• disposing a third coating containing an activity preservative over the second coating along the internal surface of the pipette tip, forming a coated pipette tip for use in performing the enzyme-linked immunosorbent assays that reduces washing time by facilitating direct pipetting into the coated pipette tip with at least one reagent mixture being selected from the group consisting of a pH stabilizer, an ionic catalyst control agent, an ionic intracellular inhibitory control agent, a detergent, a negative control agent, a chelating agent, a biocide preservative, a first antibody, and a conjugate stabilizing agent;
• and
• providing one well containing a chromogenic substrate and a visualizing reagent within a cartridge for directly reacting the chromogenic substrate and the visualizing reagent with the affinity agent in the coated pipette tip.